The highest individual concentration recorded was 496.6 ng/ml. The mean coproantigen concentration in infected herds was 13.0 ng/ml (range 0.9–112.6 ng/ml). Three groups of farms were considered according to the fasciolicide treatment: (A) flukicides were not used, (B) an anthelmintic effective against mature stages of flukes was used (albendazole or netobimin) and (C) a fasciolicide effective against immature and mature stages was used (triclabendazole: TCBZ). On the same day as the sampling, each farm owner/manager was questioned about the types of treatment used on the farm. hepatica coproantigens by a monoclonal antibody based immunoassay (MM3-COPRO ELISA). Faecal samples were taken from 5188 dairy cows on 275 randomly selected farms for measurement of the concentration of F. In this cross-sectional study, we investigated the effect of the type of flukicide treatment on the prevalence and intensity of infection in dairy cattle from Galicia, an area where fasciolosis is endemic and which is also the main milk-producing region in Spain. Effective control of fasciolosis is difficult, especially in milking cows, which can only be treated during dry periods, a control strategy that has not been yet evaluated. Fasciola hepatica infection is an important cause of lost productivity in livestock worldwide.
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